New advances in analytical and molecular technologies are having a dramatic impact on the practice of microbiology. These new and rapid methods have led to improvements in nucleic acid, chemical, immunological, and Nano/MEMs systems for the identification of microorganisms, toxins, and cellular products. These technologies are providing new and valuable solutions across multiple research and market sectors in fields such as food safety, agriculture, bio-pharmaceuticals, environmental safety, and animal health care. These emerging and rapid methods provide greater sensitivity, accuracy, precision, and reproducibility when compared to conventional growth-based methods. This workshop at the K-State–Olathe campus will provide participants an introduction into the application of these new technologies and the benefits they provide in the practice of microbiology in the real world.